In situ detection of human .BETA.1-interferon mRNA with a chemically biotinylated probe.
نویسندگان
چکیده
منابع مشابه
Detection of immunoglobulin light chain mRNA by in situ hybridisation using biotinylated tyramine signal amplification.
A highly sensitive method for the light microscopic in situ hybridisation of immunoglobulin light chain mRNA in formalin fixed, paraffin wax embedded sections is reported. This method is based on signal amplification using horseradish peroxidase catalysed deposition of biotinylated tyramine at the sites of hybridisation. kappa and lambda light chain immunoglobulin mRNA in situ hybridisation was...
متن کاملDetection of proopiomelanocortin mRNA by in situ hybridization with an oligonucleotide probe.
Synthetic oligonucleotide probes can be easily obtained and used, in contrast to cDNA cloning to develop probes, and thus the present study was carried out to determine whether such probes could also be useful for in situ hybridization. A 24-base synthetic oligonucleotide complementary to part of the alpha-melanocyte-stimulating hormone (alpha-MSH) coding region of proopiomelanocortin (POMC) mR...
متن کاملIn situ detection of calcium ions with chemically modified microcantilevers.
We report a novel technique of micromechanical detection of trace amounts of calcium ions by using microcantilevers modified with ion-selective self-assembled monolayers (SAMs). The SAM-modified microcantilevers undergo bending due to selective adsorption of calcium ions. Experiments conducted under flow conditions show that the modified cantilevers respond sensitively to calcium ions (Ca(2+));...
متن کاملIn situ hybridization using biotin-labeled oligonucleotides: probe labeling and procedures for mRNA detection.
Methods for non-radioactive in situ hybridization are rapidly progressing in refinement and application. In this overview, we present our experiences with the use of synthetic oligodeoxynucleotides, labeled with biotin- or digoxigenin-tagged nucleotides by the terminal transferase method.
متن کاملAntibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection
Antibody-based protein profiling on a global scale using immunohistochemistry constitutes an emerging strategy for mapping of the human proteome, which is crucial for an increased understanding of biological processes in the cell. Immunohistochemistry is often performed indirectly using secondary antibodies for detection, with the benefit of signal amplification. Direct immunohistochemistry ins...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1993
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.26.35